Based on over 25 years of experience and research, the SomaCount FC uses flow cytometry to accurately and rapidly enumerate the number of somatic cells in milk. Available in several versions, the SomaCount FC can be equipped with a conveyor belt, automating sampling up to 300 samples/hour.
The milk sample is first treated with an incubation reagent consisting of a clarification buffer solution and a fluorescent marker to permeate the somatic cells and stain their DNA/RNA.
After this incubation period, part of the mixture is injected into the flow cytometer in which the somatic cells are aligned and exposed to an intense laser beam to generate the fluorescence signal.
The fluorescent signal is collected by the optics, filtered and detected with a photomultiplier.
The intensity and width of the fluorescence pulses are recorded to establish the distribution curves and set the detection threshold.